FACE™ c-Jun (S63)

by Active Motif
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48625

Description

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

Each FACE c-Jun Kit provides 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total c-Jun. The S63 kit contains phospho-c-Jun antibody that recognizes c-Jun only when phosphorylated at Ser63; it does not recognize the phosphorylated forms of JunD or JunB. The S73 kit contains phospho-c-Jun antibody that recognizes c-Jun when phosphorylated at Ser73, but it also recognizes JunD when phosphorylated at Ser100. Both kits contain a total-c-Jun antibody that recognizes c-Jun regardless of the phosphorylation state. Click the c-Jun Info tab below for data and more information.

 
Name Format Cat No. Price  
FACE™ c-Jun (S63) 1 x 96 rxns 48125 $525 Buy Now
FACE™ c-Jun (S63) 5 x 96 rxns 48625 $2,100 Buy Now
FACE™ c-Jun (S63) Chemi 1 x 96 rxns 48225 $580 Buy Now
FACE™ c-Jun (S63) Chemi 5 x 96 rxns 48725 $2,320 Buy Now
FACE™ c-Jun (S73) 1 x 96 rxns 48135 $525 Buy Now
FACE™ c-Jun (S73) 5 x 96 rxns 48635 $2,100 Buy Now
FACE™ c-Jun (S73) Chemi 1 x 96 rxns 48235 $580 Buy Now
FACE™ c-Jun (S73) Chemi 5 x 96 rxns 48735 $2,320 Buy Now
Measurement of phosphorylated c-Jun (S63), phosphorylated c-Jun (S73) and total c-Jun using the FACE c-Jun (S63) and FACE c-Jun (S73) Cell Based ELISAs (In Cell Westerns).
 
Figure 1: Measurement of phosphorylated and total c-Jun.

NIH/3T3 cells were cultured in 96-well plates and serum-starved for 16 hours. Cells were then treated with 25 µg/ml of anisomycin for 30 minutes and fixed. Total and phospho c-Jun were each assayed in triplicate using either the phospho-c-Jun (S63), phospho-c-Jun (S73) or total c-Jun antibodies included in the FACE c-Jun Kit. Data was plotted after correction for cell number (performed through use of Crystal Violet).

Antibody Specificities

The phospho-c-Jun (S63) antibody is specific for phosphorylated c-Jun (S63) and was raised against a synthetic phospho-peptide corresponding to residues surrounding Serine 63 of human c-Jun. This antibody recognizes c-Jun when phosphorylated at this site and does not recognize the phosphorylated forms of JunD or JunB. The phospho-c-Jun (S73) antibody is specific for phosphorylated c-Jun (S73) and was raised against a synthetic phospho-peptide corresponding to residues surrounding Serine 73 of human c-Jun. This antibody recognizes c-Jun only when phosphorylated at this site and also recognizes JunD when phosphorylated at Serine 100. The total-c-Jun antibody recognizes c-Jun proteins regardless of the phosphorylation state. Each antibody is provi

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