Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.
FACE AKT Kits provide 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total AKT. The phospho-AKT antibody recognizes AKT1 only when phosphorylated at Ser473, and AKT2 and AKT3 only when phosphorylated at equivalent sites; the total-AKT antibody recognizes AKT1, AKT2 and AKT3 regardless of the phosphorylation state. Click the AKT Info tab below for data and more information.