FACE™ AKT

by Active Motif
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Description

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

FACE AKT Kits provide 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total AKT. The phospho-AKT antibody recognizes AKT1 only when phosphorylated at Ser473, and AKT2 and AKT3 only when phosphorylated at equivalent sites; the total-AKT antibody recognizes AKT1, AKT2 and AKT3 regardless of the phosphorylation state. Click the AKT Info tab below for data and more information.

 
Name Format Cat No. Price  
FACE™ AKT 1 x 96 rxns 48120 $525 Buy Now
FACE™ AKT 5 x 96 rxns 48620 $2,100 Buy Now
FACE™ AKT Chemi 1 x 96 rxns 48220 $580 Buy Now
FACE™ AKT Chemi 5 x 96 rxns 48720 $2,320 Buy Now
 
Measurement of phosphorylated AKT and total AKT using the FACE AKT Cell Based ELISA (In Cell Western) method.
 
Figure 1: Measurement of phosphorylated and total AKT.

NIH/3T3 cells were cultured in 96-well plates and serum-starved for 16 hours. Cells were then treated with 50 ng/ml PDGF for 5 minutes and fixed. Total and phospho-AKT were each assayed in triplicate using the phospho and total AKT antibodies from the FACE AKT Kit.

Antibody Specificities

The phospho-AKT antibody is specific for phosphorylated AKT and was raised against a phospho-Ser473 peptide that corresponds to the sequence surrounding Ser473 of mouse AKT. It recognizes AKT1 only when phosphorylated at Ser473, and AKT2 and AKT3 only when phosphorylated at equivalent sites. The total-AKT antibody recognizes A

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