FACE™ p38

by Active Motif
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48600

Description

Fast Activated Cell-based ELISA (FACE™) Kits provide a simple, sensitive method for detecting protein phosphorylation directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These 96-well, high-throughput assays are available in both colorimetric and chemiluminescent formats for over 20 different targets (see list at right). For complete details, click the FACE™ Method tab below.

FACE p38 Kits provide 96 rxns each of 2 antibodies that enable you to monitor and compare the levels of both phosphorylated and total p38. The phospho-p38 antibody recognizes the p38 alpha, beta, gamma and delta isoforms only when phosphorylated at Thr180 and Tyr182. The total-p38 antibody recognizes p38 proteins regardless of their phosphorylation states. Click the p38 Info tab below for data and more information.

 
Name Format Cat No. Price  
FACE™ p38 1 x 96 rxns 48100 $525 Buy Now
FACE™ p38 5 x 96 rxns 48600 $2,100 Buy Now
FACE™ p38 Chemi 1 x 96 rxns 48200 $580 Buy Now
FACE™ p38 Chemi 5 x 96 rxns 48700 $2,320 Buy Now
Measurement of phosphorylated p38 (T180/Y182) and total p38 using the FACE p38 Cell Based ELISA (In Cell Western) method.
 
Figure 1: Colorimetric measurement of phosphorylated p38.

Macrophage 4/4 cells were cultured in 96-well plates. Cells were induced with the indicated amount of lipopolysaccharide (LPS) for 20 minutes. Phosphorylation of p38 was assayed in triplicate using the FACE p38 Kit. Cell numbers in each well were normalized using Crystal Violet.

Antibody Specificities

The phospho-p38 antibody is specific for phosphorylated p38 and was raised against a peptide phosphorylated on residues that correspond to the sequence surrounding Thr180 and Tyr182 of human p38. It recognizes p38 alpha, beta, gamma and delta isoforms. The total-p38 antibody recognizes p38 MAPK regardless of its phosphorylation state.

p38 MAPK Overvie

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