TransAM® MAPK Family

by Active Motif
Available SKUs: 47296 |
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Description

TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.

TransAM® MAPK Family Transcription Factor ELISA Kits

TransAM MAPK Family Kits provide everything needed to study transcription factors activated by Mitogen-Activated Protein Kinase (MAPK) signal transduction pathways, including a positive control extract. The MAPK Family Kit contains multiple binding sites in each well and includes antibodies specific for the active forms of the transcription factors: phosphorylated ATF-2 (Thr71), phosphorylated c-Jun (Ser73), c-Myc, MEF2 and STAT1?, all of which are regulated by MAP kinase cascades and are key markers in cell signaling. In addition to the TransAM MAPK Family Kit, individual kits for studying ATF-2, c-Jun, c-Myc and MEF2 are also available. The phosphorylated ATF-2, MEF2 and STAT1? antibodies can be used with human extracts, while the phosphorylated c-Jun and c-Myc antibodies can be detected in human and mouse extracts. Recombinant c-Fos, Recombinant c-Jun and Recombinant c-Myc proteins are available separately to generate an optional protein standard curve in the TransAM Transcription Factor ELISA kits. Click the MAPK Info tab below for data and more information; kit manuals can be downloaded under the Documents tab.

 
Name Format Cat No. Price  
TransAM® MAPK Family 2 x 96 rxns 47296 $1,345 Buy Now

MAPK Info

The activation of Mitogen-Activated Protein Kinase (MAPK) signal transduction pathways is responsible for the phosphorylation of transcription factors by the terminal kinase in these cascades. MAPK pathways play major roles in converting mitogenic and stress stimuli into nuclear responses. At least three parallel MAPK pathways exist in humans. The extracellular signal-regulated protein kinase (ERK) pathway primarily transmits mitogenic and differentiation stimuli, while the c-Jun N-terminal kinase (JNK) and p38 pathways predominantly transmit stress and cytokine stimuli. ATF-2 (activating transcription factor 2, also known as CREB2 or cAMP responsive element binding protein 2) is a member of the ATF/CREB transcription factor family that binds to the cAMP response element (CRE). ATF-2 is activated by three MAPK pathways, ERK1/2, JNK and p38. Phosphorylation of Thr71 by MAPK14 or JNK increases transcriptional activity. MEF2, a member of the MADS box family, is mainly involved in muscle differentiation and is activated by both the p38 and ERK5 pathways. c-Myc is a cellular homolog of the avian retroviral v-myc oncogene and belongs to the Myc family, which also includes N-myc and L-myc. Translocations and deregulation of c-Myc have been implicated in cancers such as hematopoietic tumors, leukemias and

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