TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.
TransAM® MyoD Transcription Factor ELISA Kits
TransAM MyoD Kits provide everything needed to study activated Myogenic Differentiation 1 (MyoD), including a positive control extract. The MyoD Kit can be used with human, mouse and rat extracts. Click the MyoD Info tab below for data and more information; kit manuals can be downloaded under the Documents tab.
MyoD Transcription Factor Info
Myogenic differentiation1 (MyoD), also known as myogenic factor 3 (Myf-3) is a transcription factor belonging to the myogenic factors family that contains a basic helix-loop-helix (bHLH) DNA-binding domain. The MyoD transcription factor is the central target in the signaling pathways that regulate muscle development. Although MyoD family members have been studied for decades in several muscle cell systems, the overall muscle differentiation program is still a strong area of focus to understand muscle decay.
Figure 1: Monitoring MyoD activity with the TransAM MyoD Kit:
Increasing amounts of nuclear extracts from confluent C2C12 cells (filled circles) and from NIH3T3 cells (open circles) are tested for MyoD activity using the TransAM MyoD Kit.
The TransAM® transcription factor ELISA advantage
Historically, transcription factor studies have been conducted using gelshift, Western blot and reporter plasmid transfections, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. TransAM assays are up to 100 times more sensitive than gelshift techniques, and can be completed in less than 5 h