TransAM® FKHR (FOXO1)

by Active Motif
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Description

TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.

TransAM® FKHR (FOXO1) Transcription Factor ELISA Kits

TransAM FKHR (FOXO1) Kits provide everything needed to study activated Forkhead box O1, including a positive control extract. The FKHR kit can be used with human, mouse and rat extracts. Click the FKHR Info tab below for data and more information; kit manuals can be downloaded under the Documents tab.

 
Name Format Cat No. Price  
TransAM® FKHR (FOXO1) 1 x 96 rxns 46396 $685 Buy Now
TransAM® FKHR (FOXO1) 5 x 96 rxns 46896 $2,795 Buy Now

FKHR (FOXO1) Transcription Factor Info

The Forkhead transcription factor FKHR (also known as FOXO1, or Forkhead in rhabdomyosarcoma) belongs to the FOX family of transcription factors that regulate genes involved with proliferation, differentiation and longevity. While all FOX proteins contain the distinct forkhead-box motif that binds DNA, the proteins can be further grouped into subclasses.  Members of FOX class O regulate metabolism, proliferation and longevity as influenced by post-translational modifications such as phosphorylation, acetylation and ubiquitination. TransAM FKHR Kit contains an antibody specific for detection of FOXO1 in human, mouse and rat samples.

 
 
 
Figure 1: Monitoring FKHR activity with the TransAM FKHR Kit.

Different amounts of nuclear extracts from untreated Raji (purple bars) and HeLa (copper bars) cells are tested for FKHR activity using the TransAM FKHR Kit.

The TransAM® transcription factor ELISA advantage

Historically, transcription factor studies have been conducted using gelshift, Western blot and reporter plasmid transfections, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. TransAM assays are up to 100 times more sensitive than ge

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