TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.
TransAM® HNF Transcription Factor ELISA Kits
TransAM HNF Family and HNF-1 Kits provide everything needed to study activated Hepatocyte Nuclear Factors (HNFs), including a positive control extract. The HNF Family Kit measures HNF-1, HNF-3?, HNF-3? and HNF-4? from human, mouse and rat extracts. HNF-1 can be detected separately using the TransAM HNF-1 Kit with human, mouse and rat extracts. Click the HNF Info tab below for data and more information; kit manuals can be downloaded under the Documents tab.