TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.
TransAM® STAT Transcription Factor ELISA Kits
TransAM STAT Kits provide everything needed to study Signal Transducers and Activators of Transcription (STAT), including a positive control extract. TransAM STAT3 Kits are available with antibodies specific for the activated form of STAT3. A TransAM STAT Family kit is also available with the ability to independently screen the members of the STAT family (STAT1?, STAT3, STAT5A & STAT5B) simultaneously. The STAT1?, 5A and 5B antibodies can be used with human and rat extracts, while the STAT3 antibodies can be used with human, mouse and rat extracts. See the STAT Info tab below for kit data and more information; the kit manual can be downloaded under the Documents tab.