TransAM® Elk-1

by Active Motif
Available SKUs: 44896 |
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Description

TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.

TransAM® Elk-1 Transcription Factor ELISA Kits

TransAM Elk-1 Kits provide everything needed to study activated Elk-1, member of ETS oncogene family, including a positive control extract. The Elk-1 kit can be used to detect Elk-1 phosphorylated at Ser383 in human extracts. Click the Elk1 Info tab below for data and more information; kit manuals can be downloaded under the Documents  tab.

 
Name Format Cat No. Price  
TransAM® Elk-1 1 x 96 rxns 44396 $685 Buy Now
TransAM® Elk-1 5 x 96 rxns 44896 $2,795 Buy Now

Elk1 Transcription Factor Info

Elk-1, member of ETS oncogene family, is a transcription factor that is activated by mitogen-activated protein kinases (MAPKs) and whose main function is the regulation of growth-related proteins in response to extracellular stimuli. Ets family members share a Ets domain and a winged helix-loop-helix DNA binding domain that recognizes a GGAA/T-based sequence. Phosphorylation of Elk-1 increases the likelihood of ternary complex formation and binding with the serum response factor and the serum response element in the c-fos promoter. Elk-1 can be stimulated by the ERK1/2, JNK and p38 MAPK pathways to regulate the activity of the c-fos proto-oncogene in response to extracellular stimuli. Elk-1 is also a nuclear target for the ras-raf-MAPK signaling cascade. TransAM Elk-1 Kits contain antibody directed against Elk-1 phosphorylated at serine 383.

Active Motif also offers our FACE™ In-cell Western Phospho ELISAs for quantifying and comparing the phosphorylated and total amounts of ERK1/2, JNK and p38 proteins.

 
TransAM Elk-1 data 
 
Figure 1: Monitoring Elk-1 activity in different cell lines with the TransAM Elk-1 Kit.

Different amounts of nuclear extracts from untreated U-937, untreated HeLa and HeLa (TPA, 10 min) cells are tested for Elk-1 activity using the TransAM Elk-1 Kit.

The TransAM® transcription factor ELISA advantage

Historically, transcription factor studies have been conducted using gelshift, Western blot and reporter plasmid transfections, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. TransAM assays are up to 100 times more sensitive than gelshift techniques, and c

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