TransAM c-Myc Kits provide everything needed to study activated c-Myc, including a positive control extract. The c-Myc kit can be used with human and mouse extracts. TransAM c-Myc Kits contain an antibody specific for the active form of c-Myc when bound to its target DNA. Recombinant c-Myc protein is available separately to generate an optional protein standard curve. The Myc gene is a proto-oncogene that codes for a transcription factor that regulates cell growth, differentiation, glycolysis and apoptosis. c-Myc is a cellular homolog of the avian retroviral v-myc oncogene and belongs to the Myc family, which also includes N-myc and L-myc. Translocations and deregulation of c-Myc have been implicated in cancers such as hematopoietic tumors, leukemias and lymphomas. Heterodimer formation of c-Myc with Max (Myc associated factor X) is required to bind DNA at the E-box motif CAC[GA]TG and activate transcription. c-Myc is activated by the MAP Kinase ERK1/2. Because of c-Myc's regulation by MAPK, we also offer the TransAM MAPK Family Kit for studying the MAPK regulated transcription factors ATF-2, c-Jun, c-Myc, MEF2 and STAT1?. The c-Myc Respone Element is available as a pre-cloned transfection ready luciferase reporter vector on the LightSwitch™ Reporter System page under Synthetic Response Element Collection.
The TransAM® Method tab below provides complete method details; the kit manual can be downloaded under the Documents tab, while a selection of papers that cite the use of TransAM c-Myc can be seen under the Publications tab.
Measurement of c-Myc activity.