TransAM® MEF2

by Active Motif
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Description

TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.

TransAM® MEF2 Transcription Factor ELISA Kits

TransAM MEF2 Kits provide everything needed to study activated Myocyte-specific Enhancer Factor 2 (MEF2), including a positive control extract. The MEF2 kit can be used to detect the active form of MEF2 in human extracts. Click the MEF2 Info tab below for data and more information; kit manuals can be downloaded under the Documents  tab.

Because of MEF's regulation by MAPK, we also offer the TransAM MAPK Family Kit for studying the MAPK regulated transcription factors ATF-1, c-Jun, c-Myc, MEF2 and STAT1?.

 
Name Format Cat No. Price  
TransAM® MEF2 1 x 96 rxns 43196 $685 Buy Now
TransAM® MEF2 5 x 96 rxns 43696 $2,795 Buy Now

MEF2 Transcription Factor Info

Myocyte-specific enhancer factor 2 (MEF2), a member of the MADS box family, is mainly involved in muscle differentiation, but also plays roles in muscle hypertrophy, neuronal survival and T-cell apoptosis. The MEF2 family of proteins bind to target DNA sequences present in muscle-specific genes. MEF2 is activated by both the p38 and ERK5 pathways.

 

TransAM MEF2 data 
 
Figure 1: TransAM profiling of MAPK-regulated transcription factors, including MEF2.

The TransAM MAPK Family Kit was used to assay the activation levels of ATF-2, c-Myc, c-Jun, STAT1? and MEF2 using nuclear extracts prepared from either HeLa (TPA stimulated), Jurkat (unstimulated), K-562 (TPA stimulated) or U-937 (TPA + IFN? treated) cells.

The TransAM® transcription factor ELISA advantage

Historically, transcription factor studies have been conducted using gelshift, Western blot and reporter plasm

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