TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.
TransAM® MEF2 Transcription Factor ELISA Kits
TransAM MEF2 Kits provide everything needed to study activated Myocyte-specific Enhancer Factor 2 (MEF2), including a positive control extract. The MEF2 kit can be used to detect the active form of MEF2 in human extracts. Click the MEF2 Info tab below for data and more information; kit manuals can be downloaded under the Documents tab.
Because of MEF's regulation by MAPK, we also offer the TransAM MAPK Family Kit for studying the MAPK regulated transcription factors ATF-1, c-Jun, c-Myc, MEF2 and STAT1?.