TransAM® Oct-4

by Active Motif
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Description

TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.

TransAM® Oct-4 Transcription Factor ELISA Kits

TransAM Oct-4 Kits provide everything needed to study Oct-4, including a positive control extract. The kit can be used with human, mouse and rat extracts. See the Oct4 Info tab below for kit data and more information; the kit manual can be downloaded under the Documents tab.

 
Name Format Cat No. Price  
TransAM® Oct-4 1 x 96 rxns 42496 $685 Buy Now
TransAM® Oct-4 5 x 96 rxns 42996 $2,795 Buy Now

Oct-4 Transcription Factor Info

Oct-4, also known as Oct-3/4, POU5F1 or POU class 5 homeobox 1 transcription factor is a key factor involved in the first differentiation step of embryogenesis and functions to maintain an undifferentiated cell state. Oct-4 forms a trimeric complex with SOX2 on DNA; the expression of Oct-4 is associated with undifferentiated cells and tumors.

 

 
 
Figure 1: Monitoring Oct-4 activation with the TransAM Oct-4 Kit.

Different amounts of nuclear extracts from unstimulated P19 and HeLa cells are tested for Oct-4 activation using the TransAM Oct-4 Kit.

The TransAM® transcription factor ELISA advantage

Historically, transcription factor studies have been conducted using gelshift, Western blot and reporter plasmid transfections, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. TransAM assays are up to 100 times more sensitive than gelshift techniques, and can be completed in less than 5 hours. Because

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