TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.
TransAM® ATF-2 Transcription Factor ELISA Kits
TransAM ATF-2 Kits provide everything needed to study Activating Transcription Factor 2 (ATF-2) phosphorylated at threonine 71, including a positive control extract. The kit can be used with human extracts. Reactivity with other species has not been determined. Because of ATF-2's regulation by MAPK, we also offer the TransAM MAPK Family Kit for studying the MAPK regulated transcription factors ATF-2, c-Jun, c-Myc, MEF2 and STAT1?. See the ATF Info tab below for kit data and more information; the kit manual can be downloaded under the Documents tab.