TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.
TransAM® ER Transcription Factor ELISA Kits
TransAM ER Kits provide everything needed to study activated Estrogen Receptor alpha (ER?), including a positive control extract. The ER kit detects ER? from human, mouse and rat extracts. Click the ER Info tab below for data and more information; kit manuals can be downloaded under the Documents tab.
Estrogen receptors (ER) are nuclear hormone receptors that mediate the actions of the endogenous steroid hormone, 17?-estradiol (E2). ER is synthesized as two protein forms, ER? and ER?, which can bind to the estrogen receptor element (ERE) as homo- or heterodimers. Cell proliferation and differentiation triggered by ER activation has been heavily studied in bone-wasting and breast cancer treatments. Estrogen receptors are necessary for sexual development and reproductive function. The TransAM ER Kit contains an antibody specific for the active form of ER? (also known as estrogen receptor 1 or ESR1) when it is bound to the target DNA.
Figure 1: Monitoring ER? activation with the TransAM ER Kit.
Different amounts of untreated and H2O2 post-treated nuclear extracts from MCF-7 cells are tested for ER activity using the TransAM ER Kit.
The TransAM® transcription factor ELISA advantage
Historically, transcription factor studies have been conducted using gelshift, Western blot and reporter plasmid transfections, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. TransAM assays are up to 100 times more sensitive than gelshift techniques, and can be comple