TransAM® p53

by Active Motif
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TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.

TransAM® p53 Transcription Factor ELISA Kits

TransAM p53 Kits provide everything needed to study activated p53 tumor suppressor, including a positive control extract. The kit can be used with human extracts. Recombinant p53 protein is available separately to generate an optional protein standard curve in the TransAM p53 Transcription Factor ELISA kits. See the p53 Info tab below for kit data and more information; the kit manual can be downloaded under the Documents tab.

Name Format Cat No. Price  
TransAM® p53 1 x 96 rxns 41196 $685 Buy Now
TransAM® p53 5 x 96 rxns 41696 $2,795 Buy Now

p53 Transcription Factor Info

p53, also known as tumor protein p53 (TP53), is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity to control the expression of genes that regulate cell growth control, DNA repair, cell-cycle arrest, apoptosis, angiogenesis, redox regulation, metastasis, nitric oxide production and protein degradation. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Mutational inactivation of the p53 gene product is one of the most common genetic events that occur in human cancers. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, a ubiquitin ligase specific for p53, which causes its destabilization.


    Figure 1: Monitoring p53 activation with the TransAM p53 Kit.

    Different amounts of nuclear extracts from untreated (opened squares) and H2O2-treated (filled circles) MCF-7 cells are tested for p53 activation using the TransAM p53 Kit.

The TransAM® transcription factor ELISA advantage

Historically, transcription factor studies have been conducted using gelshift, Western blot and reporter plasmid transfections, which are time-consuming, do not allow for high-throughput and provide only semi-quantitative results. TransAM assays

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