TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. For complete details, click the TransAM® Method tab below.
TransAM® p53 Transcription Factor ELISA Kits
TransAM p53 Kits provide everything needed to study activated p53 tumor suppressor, including a positive control extract. The kit can be used with human extracts. Recombinant p53 protein is available separately to generate an optional protein standard curve in the TransAM p53 Transcription Factor ELISA kits. See the p53 Info tab below for kit data and more information; the kit manual can be downloaded under the Documents tab.