ATF-2 antibody (pAb)
for ATF-2 antibody (pAb)Application Notes
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for ATF-2 antibody (pAb)Immunogen
This ATF-2 antibody was raised against a synthetic peptide corresponding to amino acid residues 13-27 of human ATF-2.
for ATF-2 antibody (pAb)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for ATF-2 antibody (pAb)References
ATF-2 (activating transcription factor 2) transcription factor is a member of the leucine-zipper family of proteins and was previously known as cAMP responsive element binding protein 2 (or CREB2). ATF 2 is a transcriptional activator that binds the cAMP-responsive element (CRE) and interacts with c-Jun to stimulate CRE transcription. This protein is also capable of acetylating Histone H2B and Histone H4 in vitro as a histone acetyltransferase (HAT).
for ATF-2 antibody (pAb)Positive Control
Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for ATF-2 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for ATF-2 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting