ATF-2 antibody (pAb)

by Active Motif
Available SKUs: 40977 |
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for ATF-2 antibody (pAb)Application Notes

Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for ATF-2 antibody (pAb)Immunogen

This ATF-2 antibody was raised against a synthetic peptide corresponding to amino acid residues 13-27 of human ATF-2.

for ATF-2 antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for ATF-2 antibody (pAb)References

ATF-2 antibody (pAb) tested by Western blot.

ATF-2 pAb tested by Western blot.
ATF-2 detection by Western blot. The analysis was performed using 293 nuclear extract and ATF-2 pAb at a 2 µg/ml dilution.

for ATF-2 antibody (pAb)Background

ATF-2 (activating transcription factor 2) transcription factor is a member of the leucine-zipper family of proteins and was previously known as cAMP responsive element binding protein 2 (or CREB2). ATF 2 is a transcriptional activator that binds the cAMP-responsive element (CRE) and interacts with c-Jun to stimulate CRE transcription. This protein is also capable of acetylating Histone H2B and Histone H4 in vitro as a histone acetyltransferase (HAT).

for ATF-2 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for ATF-2 antibody.

for ATF-2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ATF-2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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