p73?N antibody (mAb)
for p73DN antibody (mAb) (Clone 38C674)Application Notes
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for p73DN antibody (mAb) (Clone 38C674)Immunogen
This p73DN antibody was raised against a peptide corresponding to amino acid residues 2-13 of human p73?N. This antibody does not cross react with full-length p73.
for p73DN antibody (mAb) (Clone 38C674)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for p73DN antibody (mAb) (Clone 38C674)References
p73?N is a member of the p53 protein family which includes p53 as well as p63. Whereas p53 transactivates the retinoblastoma gene, p63 and p73 induce the cyclin-dependent kinase inhibitor p57 to maintain Rb in an active, hypophosphorylated state. p73?N inhibits the functions of the p53 family in differentiation control, prevents myogenic differentiation, and enables cooperating oncogenes to transform myoblasts to tumorigenicity. p73?N is frequently overexpressed in rhabdomyosarcoma and is essential for tumor progression in vivo. These findings establish differentiation control as a key tumor suppressor activity of the p53 family.
for p73DN antibody (mAb) (Clone 38C674)Positive Control
Extracts from cells transfected with p73?N cDNA can be used as a positive control for p73?N antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for p73DN antibody (mAb) (Clone 38C674)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting