p73 antibody (mAb)
for p73 antibody (mAb) (Clone 5B429)Application Notes
WB: 1:500 dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for p73 antibody (mAb) (Clone 5B429)Immunogen
This p73 antibody was raised against a full-length protein corresponding to human p73. This p73 antibody reacts with an epitope that is located on the N-terminal region of human p73 and does not cross react with p53. The p73 protein may run at 63 kDa in some cell lines due to post-transcriptional modifications.
for p73 antibody (mAb) (Clone 5B429)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for p73 antibody (mAb) (Clone 5B429)References
p73 is a p53-related tumor protein, with some structural resemblance to p53, and is therefore considered a tumor-suppressor protein. p73 has numerous isoforms arising from alternative splicing, resulting in 6 different C-termini. Overexpression of p73 promotes growth arrest and/or apoptosis in a manner similar to that seen with p53.
for p73 antibody (mAb) (Clone 5B429)Positive Control
Extracts from cells transfected with p73 can be used as a positive control for p73 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for p73 antibody (mAb) (Clone 5B429)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting