DAP-5 antibody (mAb)

by Active Motif
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for DAP-5 antibody (mAb) (Clone 39C534)Application Notes

Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for DAP-5 antibody (mAb) (Clone 39C534)Immunogen

This DAP-5 antibody was raised against a synthetic peptide corresponding to amino acid residues 796-812 of human DAP-5.

for DAP-5 antibody (mAb) (Clone 39C534)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for DAP-5 antibody (mAb) (Clone 39C534)References

DAP-5 antibody (mAb) tested by Western blot.

DAP-5 mAb tested by Western blot.
DAP-5 detection by Western blot. The analysis was performed using 15 µg of Jurkat whole-cell extract and the DAP-5 mAb at a 1 µg/ml dilution.

for DAP-5 antibody (mAb) (Clone 39C534)Background

DAP-5 (eIF4G2 or eukaryotic translation initiation factor 4 gamma 2) shares similarity with the C-terminal region of eIF4G, which contains the binding sites for eIF4A and eIF3. eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. DAP-5 appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection.

for DAP-5 antibody (mAb) (Clone 39C534)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DAP-5 antibody (mAb) (Clone 39C534)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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