CREB antibody (mAb)
for CREB antibody (mAb) (Clone 82B514)Application Notes
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for CREB antibody (mAb) (Clone 82B514)Immunogen
This CREB antibody was raised against a synthetic peptide corresponding to human CREB.
for CREB antibody (mAb) (Clone 82B514)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for CREB antibody (mAb) (Clone 82B514)References
CREB (cAMP response element binding) transcription factor binds as a homodimer to specific DNA sequences called cAMP response elements (CRE) and regulates transcription. CREB belongs to the leucine zipper family of DNA binding proteins. CREB induces transcription in response to hormonal stimulation.
for CREB antibody (mAb) (Clone 82B514)Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for CREB antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for CREB antibody (mAb) (Clone 82B514)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting