Livin/ML-IAP antibody (mAb)
for Livin/ML-IAP antibody (mAb) (Clone 88C570)Application Notes
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for Livin/ML-IAP antibody (mAb) (Clone 88C570)Immunogen
This Livin/ML-IAP antibody was raised against a GST-fusion protein corresponding to human Livin/ML-IAP.
for Livin/ML-IAP antibody (mAb) (Clone 88C570)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for Livin/ML-IAP antibody (mAb) (Clone 88C570)References
Livin (ML-IAP or BIRC7) protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, Livin (ML-IAP or BIRC7) inhibits Caspase-3 and proteolytic activation of pro-Caspase-9. Isoform 1 of Livin (ML-IAP or BIRC7) blocks staurosporine-induced apoptosis, while isoform 2 blocks etoposide-induced apoptosis.
for Livin/ML-IAP antibody (mAb) (Clone 88C570)Positive Control
Extracts from cells transfected with human Livin cDNA can be used as a positive control for Livin/ML-IAP antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Livin/ML-IAP antibody (mAb) (Clone 88C570)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting