IKKi/IKK? antibody (pAb)

by Active Motif
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40957

Description

for IKKi/IKKe antibody (pAb)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IKKi/IKKe antibody (pAb)Immunogen

This IKKi/IKK? antibody was raised against a synthetic peptide corresponding to amino acid residues 175-188, 525-540, and 567-580 of human IKKi/IKK?.

for IKKi/IKKe antibody (pAb)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IKKi/IKKe antibody (pAb)References

 
IKKi/IKK? antibody (pAb) tested by Western blot.

IKKi/IKK? pAb tested by Western blot.
IKKi/IKK? detection by Western blot. The analysis was performed using 10 µg of whole-cell extract from Daudi cells and IKKi/IKK? pAb at a 2 µg/ml dilution.

for IKKi/IKKe antibody (pAb)Background

IKKi/IKK?I?B Kinase-epsilon (also called IKKi) is a homolog of the IKK (I?B kinase) catalytic subunits IKK? and IKK?. IKK? is primarily expressed in immune cells and may be responsible for modulation of NF?B (NF?B p50 and NF?B p65) activity in the immune system.

for IKKi/IKKe antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IKKi/IKKe antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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