TLR8 antibody (mAb)
for TLR8 antibody (mAb) (Clone 44C143)Application Notes
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for TLR8 antibody (mAb) (Clone 44C143)Immunogen
This TLR8 antibody was raised against a peptide corresponding to amino acid residues 750-850 of human TLR8.
for TLR8 antibody (mAb) (Clone 44C143)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for TLR8 antibody (mAb) (Clone 44C143)References
TLR8 – Toll-Like receptor 8 is a member of the Toll-like receptor (TLR) family that plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize specific molecular features associated with pathogens, and mediate the production of cytokines necessary for the development of effective immunity. Many of the TLRs heterodimerize with each other to modulate receptor function. TLR8 expression cells is elevated after exposure to both Gram-positive and Gram-negative bacteria. While primarily extracellular, TLR8 may be also localized intracellularly.
for TLR8 antibody (mAb) (Clone 44C143)Positive Control
Extracts from 293 cells transfected with human TLR8 cDNA can be used as a positive control for TLR8 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for TLR8 antibody (mAb) (Clone 44C143)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting