D4-GDI antibody (mAb)
for D4-GDI antibody (mAb) (Clone 97A1015)Application Notes
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for D4-GDI antibody (mAb) (Clone 97A1015)Immunogen
This D4-GDI antibody was raised against a synthetic peptide corresponding to amino acid residues in the Fas-induced cleavage site of human D4-GDI.
for D4-GDI antibody (mAb) (Clone 97A1015)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for D4-GDI antibody (mAb) (Clone 97A1015)References
D4-GDI (Rho GDP dissociation inhibitor beta or ARHGDIB) plays an important role in the activation of the superoxide (O2-)-generating NADPH oxidase of phagocytes. The mechanisms involving cleavage of D4-GDI (Rho GDP) with apoptosis are not presently known. However, activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for D4-GDI antibody (mAb) (Clone 97A1015)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting