D4-GDI antibody (mAb)

by Active Motif
Available SKUs: 40941 |
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for D4-GDI antibody (mAb) (Clone 97A1015)Application Notes

Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for D4-GDI antibody (mAb) (Clone 97A1015)Immunogen

This D4-GDI antibody was raised against a synthetic peptide corresponding to amino acid residues in the Fas-induced cleavage site of human D4-GDI.

for D4-GDI antibody (mAb) (Clone 97A1015)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for D4-GDI antibody (mAb) (Clone 97A1015)References

D4-GDI antibody (mAb) tested by Western blot.

D4-GDI mAb tested by Western blot.
Detection of D4-GDI by Western blot analysis. HL-60 cells were treated for different time periods with 10 ng/ml of Fas monoclonal antibody. Harvested cells were lysed, resolved by SDS-PAGE and transferred onto a PVDF membrane. The membrane was incubated with the D4-GDI mAb and immunoreactivity was detected by chemiluminescence. The data shows that the D4-GDI antibody specifically detects the 23 kDa cleaved form of D4-GDI in a time-sensitive manner.

for D4-GDI antibody (mAb) (Clone 97A1015)Background

D4-GDI (Rho GDP dissociation inhibitor beta or ARHGDIB) plays an important role in the activation of the superoxide (O2-)-generating NADPH oxidase of phagocytes. The mechanisms involving cleavage of D4-GDI (Rho GDP) with apoptosis are not presently known. However, activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

for D4-GDI antibody (mAb) (Clone 97A1015)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for D4-GDI antibody (mAb) (Clone 97A1015)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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