Caspase-9 antibody (pAb) (Pro-form)
for Caspase-9 antibody (pAb) (Pro-form)Application Notes
WB: 1:1,000 dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for Caspase-9 antibody (pAb) (Pro-form)Immunogen
This Caspase-9 antibody (Pro-form) was raised against a peptide corresponding to amino acid residues 299 -318 of human Pro-Caspase-9.
for Caspase-9 antibody (pAb) (Pro-form)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for Caspase-9 antibody (pAb) (Pro-form)References
Caspase-9 (CASP9 or apoptosis-related cysteine peptidase 9) (Pro-form) is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-9 is processed by caspase APAF1, which is thought to be one of the earliest events in the caspase activation cascade. Caspase-9 is then involved in processing of Caspase-3.
for Caspase-9 antibody (pAb) (Pro-form)Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Caspase-9 antibody (Pro-form).
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Caspase-9 antibody (pAb) (Pro-form)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting