Caspase-8 antibody (mAb)
for Caspase-8 antibody (mAb) (Clone 90A992)Application Notes
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for Caspase-8 antibody (mAb) (Clone 90A992)Immunogen
This Caspase-8 antibody was raised against a synthetic peptide corresponding to amino acid residues 217-222 of human Caspase-8.
for Caspase-8 antibody (mAb) (Clone 90A992)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for Caspase-8 antibody (mAb) (Clone 90A992)References
Caspase-8 (CASP8 or apoptosis-related cysteine peptidase 8) is the most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adaptor molecule FADD recruits it to either receptor. Caspase-8 cleaves and activates Caspase-3, Caspase-4, Caspase-6, Caspase-7, Caspase-9 and Caspase-10.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Caspase-8 antibody (mAb) (Clone 90A992)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting