Caspase-7 antibody (mAb)
for Caspase-7 antibody (mAb) (Clone 25B204.1)Application Notes
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for Caspase-7 antibody (mAb) (Clone 25B204.1)Immunogen
This Caspase-7 antibody was raised against a recombinant human Caspase-7 protein.
for Caspase-7 antibody (mAb) (Clone 25B204.1)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for Caspase-7 antibody (mAb) (Clone 25B204.1)References
Caspase-7 (CASP7 or apoptosis-related cysteine peptidase 7) is involved in the activation cascade of caspases responsible for apoptosis execution. The precursor form of Caspase-7 is cleaved by Caspase-3 and Caspase-10, which is activated by cell death stimuli and induces apoptosis. Caspase-7 cleaves and activates sterol regulatory element binding proteins (SREBPs) and proteolytically cleaves poly(ADP-ribose) polymerase (PARP, see PARP-1 C-terminal and PARP-1 N-terminal antibodies).
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Caspase-7 antibody (mAb) (Clone 25B204.1)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting