Caspase-3 antibody (mAb)

by Active Motif
Available SKUs: 40924 |
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for Caspase-3 antibody (mAb) (Clone 31A1067)Application Notes

Validated Applications:
WB: 2 - 4 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. >

Published Applications:

for Caspase-3 antibody (mAb) (Clone 31A1067)Immunogen

This Caspase-3 antibody was raised against a 6xHis-Tag full-length human Caspase-3 protein.

for Caspase-3 antibody (mAb) (Clone 31A1067)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for Caspase-3 antibody (mAb) (Clone 31A1067)References

Caspase-3 antibody (mAb) tested by Western blot.

Caspase-3 mAb tested by Western blot.
Western blot analysis for detection of Caspase-3 activation. HeLa cells were treated with 2 µM staurosporine for different time periods and then probed using Caspase-3 mAb. Caspase-3 activation is determined by cleavage of pro-Caspase-3, which generates 17 and 12 kDa proteins, the larger and smaller catalytic subunit, respectively.

for Caspase-3 antibody (mAb) (Clone 31A1067)Background

Caspase-3 (CASP3 or apoptosis-related cysteine peptidase 3) is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-3 cleaves and activates Caspase-6, Caspase-7 and Caspase-9, and the protein itself is processed by Caspase-8, Caspase-9 and Caspase-10. Caspase-3 is the predominate caspase involved in the cleavage of amyloid-beta 4A, which is associated with Alzheimer's disease.

for Caspase-3 antibody (mAb) (Clone 31A1067)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Caspase-3 antibody.

for Caspase-3 antibody (mAb) (Clone 31A1067)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Caspase-3 antibody (mAb) (Clone 31A1067)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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