Caspase-3 antibody (mAb)
for Caspase-3 antibody (mAb) (Clone 31A1067)Application Notes
WB: 2 - 4 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. >
for Caspase-3 antibody (mAb) (Clone 31A1067)Immunogen
This Caspase-3 antibody was raised against a 6xHis-Tag full-length human Caspase-3 protein.
for Caspase-3 antibody (mAb) (Clone 31A1067)Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
for Caspase-3 antibody (mAb) (Clone 31A1067)References
Caspase-3 (CASP3 or apoptosis-related cysteine peptidase 3) is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-3 cleaves and activates Caspase-6, Caspase-7 and Caspase-9, and the protein itself is processed by Caspase-8, Caspase-9 and Caspase-10. Caspase-3 is the predominate caspase involved in the cleavage of amyloid-beta 4A, which is associated with Alzheimer's disease.
for Caspase-3 antibody (mAb) (Clone 31A1067)Positive Control
Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Caspase-3 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Caspase-3 antibody (mAb) (Clone 31A1067)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting