TRAF5 antibody (mAb)

by Active Motif
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for TRAF5 antibody (mAb) (Clone 55A219)Application Notes

Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for TRAF5 antibody (mAb) (Clone 55A219)Immunogen

This TRAF5 antibody was raised against a fusion protein corresponding to amino acid residues 77 to 186 of human TRAF5.

for TRAF5 antibody (mAb) (Clone 55A219)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for TRAF5 antibody (mAb) (Clone 55A219)References

TRAF5 antibody (mAb) tested by Western blot.

TRAF5 mAb tested by Western blot.
TRAF5 detection by Western blot. The analysis was performed using 10 µg HeLa nuclear extract and TRAF5 mAb at a 2 µg/ml dilution.

for TRAF5 antibody (mAb) (Clone 55A219)Background

TRAF5 (TNF receptor-associated factor 5, RNF84) scaffold protein is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain, a RING-type zinc finger, and two TRAF-type zinc fingers. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. TRAF5 is one of the components of a multiple protein complex that binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. TRAF5 mediates activation of NF?B (NF?B p65 and NF?B p50) and probably JNK. It also appears to be involved in apoptosis.

for TRAF5 antibody (mAb) (Clone 55A219)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for TRAF5 antibody.

for TRAF5 antibody (mAb) (Clone 55A219)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TRAF5 antibody (mAb) (Clone 55A219)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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