I?B? phospho Ser32,36 antibody (mAb)

by Active Motif
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40904

Description

for IkBa phospho Ser32,36 antibody (mAb) (Clone 39A1431)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IkBa phospho Ser32,36 antibody (mAb) (Clone 39A1431)Immunogen

This I?B? phospho Ser32,36 antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human I?B?.

for IkBa phospho Ser32,36 antibody (mAb) (Clone 39A1431)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IkBa phospho Ser32,36 antibody (mAb) (Clone 39A1431)References

 
I?B? phospho Ser32,36 antibody (mAb) tested by Western blot.

I?B? phospho Ser32,36 mAb tested by Western blot.
Jurkat cells were treated for 30 minutes with 100 µg/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal), a calpain and proteasome inhibitor that prevents I?B? dephosphorylation, followed by incubation with (lanes 2 & 4) or without 1 nM TNF-? (1 & 3). The membranes were blotted with the anti-phospho-I?B? (lanes 1 & 2) or anti-I?B?, which recognizes both phosphorylated and non-phosphorylated forms of I?B?. The data shows that I?B? phospho Ser32,36 mAb detects specifically the phosphorylated forms of I?B?.

for IkBa phospho Ser32,36 antibody (mAb) (Clone 39A1431)Background

I?B? phospho Ser32,36 – NF?B (NF?B p50 and NF?B p65) signaling is controlled to a large extent by the sequestration of the NF?B complex in the cytoplasm by its association with one of the I?B family of proteins. I?B? is phosphorylated at Ser32 and Ser36 by the I?B Kinase (IKK) complex, resulting in the degradation of I?B and the nuclear translocation of NF?B.

for IkBa phospho Ser32,36 antibody (mAb) (Clone 39A1431)Positive Control

Extracts from Jurkat cells treated for 30 minutes with 100 µg/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal), followed by incubation with or without 1 nM TNF-? cell extract can be used as a positive control for I?B? phospho Ser32, 36 antibody.

for IkBa phospho Ser32,36 antibody (mAb) (Clone 39A1431)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IkBa phospho Ser32,36 antibody (mAb) (Clone 39A1431)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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