I?B? antibody (mAb)

by Active Motif
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40903

Description

for IkBa antibody (mAb) (Clone 6A920)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

Published Applications:
WB

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for IkBa antibody (mAb) (Clone 6A920)Immunogen

This I?B? antibody was raised against a recombinant protein corresponding to amino acid residues 32-291 of human I?B?.

for IkBa antibody (mAb) (Clone 6A920)Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

for IkBa antibody (mAb) (Clone 6A920)References

 
I?B? antibody (mAb) tested by Western blot.

I?B? mAb tested by Western blot.
The anti-I?B? detects both phosphorylated and non-phosphorylated forms of I?B? by Western blot. Jurkat cells (~1 x 107) were treated for indicated time periods with 10 nm/ml PMA, 1 µM ionomycin and anti-CD28 (1:10,000 dilution). 10 µl of cytoplasmic (C) and nuclear extract (N) were resolved on a 8.75% SDS-PAGE and transferred to Immobilon membrane. Figure is courtesy of Dr. Shao-Cong Sun at Penn State Univ. College of Medicine.

for IkBa antibody (mAb) (Clone 6A920)Background

I?B? – NF?B (NF?B p50 and NF?B p65) signaling is controlled to a large extent by the sequestration of the NF?B complex in the cytoplasm by its association with one of the I?B family of proteins. I?B? is phosphorylated at Ser32 and Ser36 (I?B? phospho Ser32,36) by the I?B Kinase (IKK) complex, resulting in the degradation of I?B and the nuclear translocation of NF?B.

for IkBa antibody (mAb) (Clone 6A920)Positive Control

Active Motif's Jurkat nuclear extract (TPA + CI stimulated) (Catalog No. 36013) can be used as a positive control for I?B? antibody.

for IkBa antibody (mAb) (Clone 6A920)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for IkBa antibody (mAb) (Clone 6A920)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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