MCF-7 nuclear extract (H2O2 treated)

by Active Motif
Available SKUs: 40800 |
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for MCF-7 nuclear extract (H2O2 treated)Contents

2 x 100 µg of MCF-7 nuclear extract (H2O2 treated) at 2.5 µg/µl.

for MCF-7 nuclear extract (H2O2 treated)Background

MCF-7 nuclear extract (H2O2 treated) was prepared from cell cultures of the human MCF-7 breast adenocarcinoma cancer cell line. The MCF-7 cell line was originally derived from a pleural effusion of a 69-year old Caucasian woman with metastatic breast cancer. MCF-7 cells are the best characterized and most commonly utilized cell lines in in vitro breast cancer studies. The MCF-7 cell line has retained several characteristics that are particular to mammary epithelium, including the ability to grow as a monolayer, form domes, and respond to hormones. These cells also display a low metastatic potential, leading to the assumption that they represent an early epithelial adenocarcinoma phenotype. These characteristics make MCF-7 cells an ideal model system to study malignant progression in relation to breast cancer.

Treatment of MCF-7 cells with reactive oxygen species (ROS), such as H2O2 triggers an oxidative stress response that leads to toxicity and eventual apoptosis. H2O2 signals an antiproliferative/repair response that induces antioxidant systems to metabolize H2O2 to avert ROS toxicity.

for MCF-7 nuclear extract (H2O2 treated)Application Notes

MCF-7 nuclear extract (H2O2 treated) is recommended for studies related to 1) breast cancer research, 2) cellular response to oxidative stress and 3) apoptosis.

for MCF-7 nuclear extract (H2O2 treated)Extract Origin

Human breast carcinoma

for MCF-7 nuclear extract (H2O2 treated)Extract Composition

MCF-7 nuclear extract in collected in Lysis Buffer after a 3-hr incubation with 0.2 mM H2O2. The Lysis Buffer consists of 20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT. The protein content has been determined by a Bradford-based assay.

for MCF-7 nuclear extract (H2O2 treated)Quality Control

Each lot has been tested for p53 activation by using TransAM® p53 Kit. The signal intensity for p53 activation in each lot is compared to the signal intensity obtained with extracts from untreated MCF-7 cells (see figure). Once the signals are blanked, the ratio between the signals from extracts of treated cells over untreated cells used at 5 µg/well must be above 4. This ratio may vary depending of basal level of p53 activation in a given cell type.

Figure 1: Monitoring p53 activation with the TransAM p53 Kit.

Figure 1: Monitoring p53 activation with the TransAM p53 Kit.
Different amounts of nuclear extracts from untreated (opened squares) and H2O2-treated (filled circles) MCF-7 cells are tested for p53 activation using the TransAM p53 Kit.

for MCF-7 nuclear extract (H2O2 treated)Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for MCF-7 nuclear extract (H2O2 treated)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

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