THP-1 nuclear extract (TPA stimulated)
for THP-1 nuclear extract (TPA stimulated)Contents
2 x 100 µg of THP-1 nuclear extract (PMA stimulated) at 2.5 µg/µl.
for THP-1 nuclear extract (TPA stimulated)Background
THP-1 (TPA stimulated) nuclear extract was prepared from a cell culture of the human acute monocytic leukemia THP-1 cell line. The THP-1 cell line was originally derived from the peripheral blood of a 1 year old body with acute monocytic leukemia. THP-1 cells share similar properties to human monocyte-derived macrophages. These cells differentiate into mature macrophages (phagocytes) when grown in the presence of DMSO or phorbol 12-myristate 13-acetate (TPA, also known as PMA). When stimulated with DMSO or vitamin D3, THP-1 cells adhere to the walls of cell culture flasks, and are therefore frequently utilized as in vitro model systems to study foam cell formation in relation to atherosclerosis. The THP-1 cell line is characterized by the expression of Fc and C3b receptors, the production of IL-1, and the lack expression of cytoplasmic and surface immunoglobulins. In addition, these cells are phagocytic, produce lysosymes and stain positive for alpha-napthyl butyrate esterase. Because of these properties, they are often used in research to study monocyte differentiation.
for THP-1 nuclear extract (TPA stimulated)Application Notes
THP-1 nuclear extract (TPA stimulated) is recommended for use in studies related to 1) blood monocytes, 2) monocyte differentiation and 3) atherosclerosis.
for THP-1 nuclear extract (TPA stimulated)Extract Origin
for THP-1 nuclear extract (TPA stimulated)Extract Composition
THP-1 nuclear extract was collected in Lysis Buffer after a 24-hour incubation with PMA (10 ng/ml). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM ?-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.
for THP-1 nuclear extract (TPA stimulated)Quality Control
Extracts have been quality control tested by Western blot.
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
for THP-1 nuclear extract (TPA stimulated)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.