PC-12 nuclear extract (Forskolin stimulated)
for PC-12 nuclear extract (Forskolin stimulated)Contents
2 x 100 µg of PC-12 nuclear extract (Forskolin stimulated) at 2.5 µg/µl.
for PC-12 nuclear extract (Forskolin stimulated)Background
PC-12 nuclear extract (Forskolin stimulated) was prepared from cell cultures of the rat adrenal pheochromocytoma PC-12 cell line. This cell line originated from a catecholamine-secreting tumor (pheochromocytoma) of the rat adrenal medulla. In addition to catecholamine, PC-12 cells also produce neurotransmitters. PC-12 cells are frequently used as a model system for neuronal differentiation because of their ability to differentiate into a sympathetic neuronal phenotype in response to nerve growth factor, fibroblast growth factor and, under certain conditions, epidermal growth factor.
Forskolin is a diterpene compound that activates adenylyl cyclase, thus elevating intracellular levels of cAMP. Activation of the cAMP signaling pathway is associated with the regulation of a number of cellular processes, including motility, neurite outgrowth, metabolism, transcription, and neurotransmitter secretion. Similar to nerve growth factor stimulation of the MAPK signaling pathway, forskolin stimulation leads to PKA-dependent phosphorylation and activation of the CREB transcription factor. In the brain, CREB function is primarily associated with long-term potentiation in the formation of memories. Disturbance of CREB function in the brain is linked to the progression of Huntington's Disease and Rubinstein-Taybi syndrome, as well as some forms of cancer.
for PC-12 nuclear extract (Forskolin stimulated)Application Notes
PC-12 nuclear extract (Forskolin stimulated) is specifically recommended for studies related to 1) neuronal development, 2) neurobiology, 3) neurobiochemistry, and 4) neurotoxicity.
for PC-12 nuclear extract (Forskolin stimulated)Extract Origin
Rat adrenal gland; pheochromocytoma (kidney tumor)
for PC-12 nuclear extract (Forskolin stimulated)Extract Composition
PC12 nuclear extract was collected in Lysis Buffer after a 30-minute incubation with forskolin (20 µM). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM ?-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.
for PC-12 nuclear extract (Forskolin stimulated)Quality Control
Extracts have been quality control tested by Western blot and the TransAM® Transcription Factor ELISA Kit.
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
for PC-12 nuclear extract (Forskolin stimulated)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.