HeLa whole-cell extract (TNF-? stimulated)
for HeLa whole-cell extract (TNF-a stimulated)Contents
2 x 125 µg of HeLa whole-cell extract (TNF-? stimulated) at 2.5 µg/µl.
for HeLa whole-cell extract (TNF-a stimulated)Background
HeLa whole-cell extract (TNF-? stimulated) was prepared from a cell culture of the HeLa human epithelial carcinoma cell line. The HeLa cell line was originally derived from cells obtained in 1951 from Henrietta Lacks, a cervical cancer patient who eventually died from her cancer. The HeLa cell line is one of the oldest and most commonly utilized immortal cell lines in scientific research. These cells are highly prolific and easily sustainable, allowing for flexibility in their application in various fields of scientific research, including cancer biology, virology, and human disease. To date, over 60,000 publications across various disciplines have referenced the use of HeLa cells.
Stimulation of HeLa cells with the tumor necrosis factor-alpha (IL-1?), a member of a group of cytokines that stimulate the acute phase reaction of the immune response, leads to programmed cell death, or apoptosis, and induction of activation of the NF?B signal transduction pathway. The transcription factor NF?B is widely studied due to its implication in the regulation of genes that control inflammation, cell proliferation and cell survival.
for HeLa whole-cell extract (TNF-a stimulated)Application Notes
HeLa whole-cell extract (TNF-? stimulated) is specifically recommended for analysis of apoptosis and the role of NF?B activation in 1) inflammation, 2) cell growth, and 3) cell survival.
for HeLa whole-cell extract (TNF-a stimulated)Extract Origin
Human Cervix Adenocarcinoma
for HeLa whole-cell extract (TNF-a stimulated)Extract Composition
HeLa whole-cell extract was collected in Lysis Buffer after a 30-minute incubation with TNF-? (20 ng/ml). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20% glycerol, 1% Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA. The protein content has been determined by a Bradford-based assay.
for HeLa whole-cell extract (TNF-a stimulated)Quality Control
Each lot has been tested for NF?B activation by using TransAM® NF?B Kits. The signal intensity for NF?B activation in each lot is compared to the signal intensity obtained with extracts from unstimulated HeLa cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NF?B activation in a given cell type.
To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.
for HeLa whole-cell extract (TNF-a stimulated)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.