Histone Extraction Kit
The Histone Extraction Kit provides a simple method to acid precipitate histones from cell culture, tissue and primary cells (e.g. T-cells) while preserving histone post-translational modifications (PTMs). The highly basic nature of histone proteins often precludes them from isolation using traditional methods for protein extraction. Instead, histones must be isolated under acidic conditions to retain solubility. The inclusion of inhibitors helps to preserve histone modifications such as acetylation, methylation and phosphorylation.
Each kit contains enough reagents for extractions from 2 x 96-well plates (192 rxns), or 100 rxns from cultured cell lines and tissues. Kits include buffers, protease inhibitors, phosphatase inhibitors, deacetylase inhibitors and optimized protocols for efficient recovery of core histones (H2A, H2B, H3 & H4).
Lysates prepare using the Histone Extraction Kit are compatible with downstream analysis by Western blot and Histone Modification ELISA. For researchers interested in a more purified histone lysate, Active Motif also offers Histone Purification Kits:
- Histone Modification ELISAs – quantitative ELISA to measure changes in histone modification levels across samples.
- Histone Purification Kits – include additional column purification and precipitation steps for a more pure histone sample.
- Histone Antibodies – validated and verified histone PTM-specific antibodies for use in Western blot and ChIP.