Histone Purification Microplate Kit
Active Motif's Histone Purification Microplate Kit enables purification of core histones (H2A, H2B, H3 and H4) using very small amounts (as little as 0.9 x 105 cells) of starting material, and its microplate spin column format enables elution into a 96-well plate to easily transition into downstream high-throughput applications.
Achieve better yields with Histone Microplate Purification than with crude extraction.
The Histone Purification Microplate Kit method utilizes the identical proprietary buffer system used in our Histone Purification Kit and our Histone Purification Mini Kit to purify core histones from cells and tissue samples. This purification method is superior to acid precipitation methods because it preserves post-translational histone modifications and greatly improves the purity and yield of your isolated histones to improve the reliability and consistency of your sample preparation. Histones isolated by this method are excellent substrates for use in downstream high-throughput screening assays, Western blot or mass spectrometry.
- Only commercial kits available for histone purification
- Higher quality sample prep than crude acid extraction
- 96-well format with modular stripwell design for flexibility in throughput
- Preserves modifications on histones
- Purify histones from as few as 0.9x105 cells
- Works with cell or tissue samples
To learn more about the Histone Purification Microplate Kit, click on the Method or Data, or tabs below. To view a manual or other related documents, click on the Documents tab. For a complete list of reagents included in the Histone Purification Microplate Kit, please select the Contents tab below. Inhibitors are NOT included in the Histone Purification Microplate kit and are generally not required to preserve histone post-translational modifications using this kit. If inhibitors are desired, they must be supplied by the user and can be added to the extraction and neutralization buffers during isolation. Additional Extraction Buffer and 5X Neutralization buffer may be purchased separately.