Histone Purification Kit
Now you can easily purify histones and further separate the fractions of core histones from any cell culture or tissue sample while maintaining post-translational modifications like acetylation, methylation and phosphorylation states. The Histone Purification Kit enables you to isolate core histones from any cell culture or tissue sample. The core histones may be purified using the convenient spin column format as one total population containing H2A, H2B, H3 and H4, or further purified into separate fractions of H2A/H2B dimers and H3/H4 tetramers with the efficient gravity flow protocol.
To learn more about the Histone Purification Kit, click on the Description tab below. To view a manual or other related documents, click on the Documents tab. For a complete list of reagents included in the Histone Purification Kit, please select the Contents tab below. Inhibitors are NOT included in the Histone Purification kit and are generally not required to preserve histone post-translational modifications using this kit. If inhibitors are desired, they must be supplied by the user and can be added to the extraction and neutralization buffers during isolation. Additional Extraction Buffer and 5X Neutralization buffer may be purchased separately.