CoREST antibody (pAb), sample

by Active Motif
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39956

Description

for CoREST antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for CoREST antibody (pAb)Immunogen

This CoREST antibody was raised against a synthetic peptide derived from the C-terminal region of human CoREST.

for CoREST antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CoREST antibody (pAb)References

 
CoREST antibody (pAb) tested by Western blot.

CoREST antibody (pAb) tested by Western blot.
Nuclear extract of HEK293 cells (25 µg) probed with CoREST antibody (1:500 dilution).

for CoREST antibody (pAb)Background

CoREST (REST corepressor 1, RCOR1) is a corepressor protein recruited by the REST, a neuronal gene-specific transcriptional repressor, to help repress neuron specific gene expression in non-neuronal cell lineages. CoREST is an essential component of the BHC repressor complex that also contains HDAC1, HDAC2, HMG20B/BRAF35, LSD1 (a lysine-4 specific histone demethylase) and PHF21A/BHC80. The Herpes Simplex virus ICP0 protein blocks the repression of viral genes by disrupting the interaction between CoREST and HDAC1/2.

for CoREST antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for CoREST antibody.

for CoREST antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CoREST antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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