SIRT6 antibody (pAb), sample

by Active Motif
Available SKUs: 39912 |
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for SIRT6 antibody (pAb)Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Published Applications:

for SIRT6 antibody (pAb)Immunogen

This SIRT6 antibody was raised against a peptide within the C-terminus of human SIRT6.

for SIRT6 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SIRT6 antibody (pAb)References

SIRT6 antibody (pAb) tested by immunoprecipitation.

SIRT6 antibody (pAb) tested by Immunoprecipitation.
10 µl of SIRT6 antibody was used to immunoprecipitate SIRT6 from 500 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SIRT6 antibody at a dilution of 1:1,000.

SIRT6 antibody (pAb) tested by Western blot.

SIRT6 antibody (pAb) tested by Western blot.
HeLa cells nuclear extract (25 ?g) probed with SIRT6 antibody (1:1,000).

for SIRT6 antibody (pAb)Background

SIRT6 (Sir2 homologue 6) is an NAD+ dependant histone deacetylase (HDAC) related to SIRT1 and to the yeast Sir2 protein. SIRT6, originally identified as a tubulin deacetylase, it was recently found to be able to deacetylate histone H3 acetylated at lysine 9.

for SIRT6 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SIRT6 antibody.

for SIRT6 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SIRT6 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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