DNMT3L antibody (pAb)

by Active Motif
Available SKUs: 39907 |
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for DNMT3L antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for DNMT3L antibody (pAb)Immunogen

This DNMT1 antibody was raised against a recombinant protein corresponding to full-length human DNMT3L.

for DNMT3L antibody (pAb)Buffer

Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.

for DNMT3L antibody (pAb)References

DNMT3L antibody (pAb) tested by Western blot.

DNMT3L antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of human testicular cells probed with DNMT3L antibody at a dilution of 1:500.

for DNMT3L antibody (pAb)Background

DNMT3L (DNA methyltransferase 3-like) is a nuclear protein with similarity to the DNMT family of proteins, but does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation. DNMT3L is expressed in the presumptive gametes in mammalian embryos and is required for the establishment of genomic imprinting. DNMT3L has a PHD-like domain that allows binding to chromatin through interactions with the amino terminal tail of histone H3 that is devoid of lysine 4 methylation.

for DNMT3L antibody (pAb)Positive Control

Active Motif's Human Testes nuclear extract (Catalog No. 36047) can be used as a positive control for DNMT3L antibody.

for DNMT3L antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DNMT3L antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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