PGC1-? antibody (pAb), sample

by Active Motif
Available SKUs: 39860 |
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for PGC1-b antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for PGC1-b antibody (pAb)Immunogen

This PGC1-? antibody antibody was raised against a peptide within the N-terminal region of human PGC1-?.

for PGC1-b antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PGC1-b antibody (pAb)References

PGC1-? antibody (pAb) tested by Western blot.

PGC1-? antibody tested by Western blot.
Western blot of K-562 nuclear extract (40 µg/lane) probed with PGC1-? antibody (pAb) (1:500 dilution).

for PGC1-b antibody (pAb)Background

PGC1-? (Peroxisome proliferator-activated receptor gamma coactivator 1-beta, PPARGC1B, PERC) is a co-activator of transcription by nuclear receptors and transcription factors. PGC1-? participates in modulating the transcriptional activity of PPAR alpha and gamma, ER alpha, NRF1 and GR. It is likely involved in fat oxidation, non-oxidative glucose metabolism and in the regulation of energy expenditure. Expression of PGC1-? is repressed by saturated fatty acids in skeletal muscle cells and induced by insulin. PGC1-? is down-regulated in type 2 diabetes as well as in pre-diabetics. Allelic variation of PGC1-? may contribute to the pathogenesis of obesity, with the widespread Ala-203 variation being a significant risk factor for the development of this diabetes.

for PGC1-b antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for PGC1-? antibody.

for PGC1-b antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PGC1-b antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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