GCN5 antibody (pAb), sample

by Active Motif
Available SKUs: 39832 |
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for GCN5 antibody (pAb)Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution

for GCN5 antibody (pAb)Immunogen

This GCN5 antibody was raised against a peptide within the N-terminal region of human GCN5.

for GCN5 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for GCN5 antibody (pAb)References

GCN5 antibody (pAb) tested by immunoprecipitation.

GCN5 antibody (pAb) tested by Immunoprecipitation.
10 µl of GCN5 antibody was used to immunoprecipitate GCN5 from 500 µg of MCF-7 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the GCN5 antibody at a dilution of 1:500.

GCN5 antibody (pAb) tested by Western blot.

GCN5 antibody (pAb) tested by Western blot.
Detection of GCN5 by Western blot analysis. Nuclear extract of MCF-7 cells (30 µg) probed with GCN5 antibody at a dilution of 1:500.

for GCN5 antibody (pAb)Background

GCN5 (General control of nutrition 5, KAT2A) is a histone acetyltransferase (HAT) and serves as a transcriptional activator. GCN5 is a component of the SAGA and ATAC complexes, which have histone acetyltransferase activity for histones H3 and H4. GCN5 is required for neural tube closure and embryonic development.

for GCN5 antibody (pAb)Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for GCN5 antibody.

for GCN5 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for GCN5 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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