HNF-3? / FOXA2 antibody (pAb), sample

by Active Motif
Available SKUs: 39828 |
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for HNF-3b / FOXA2 antibody (pAb)Application Notes

Validated Applications:
WB: 1:2,000 - 1:5,000 dilution

Published Applications:
See references for more information. Individual optimization may be required.

for HNF-3b / FOXA2 antibody (pAb)Immunogen

This HNF-3? / FOXA2 antibody was raised against a recombinant protein corresponding to amino acids 7-86 of rat HNF-3? /FOXA2.

for HNF-3b / FOXA2 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for HNF-3b / FOXA2 antibody (pAb)References

HNF-3? / FOXA2 antibody (pAb) tested by Western blot.

HNF-3? / FOXA2 antibody (pAb) tested by Western blot.
Extract of HeLa cells expressing HNF-3? / FOXA2 (50 µg per lane) probed with HNF-3? / FOXA2 antibody (pAb) (1:5,000 dilution).

for HNF-3b / FOXA2 antibody (pAb)Background

HNF-3? (Hepatocyte nuclear factor 3 beta, forkhead box A2, FoxA2) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1?, HNF-1?, HNF-3?, HNF-3?, HNF-3?, HNF-4?, HNF-4?, HNF-6? and HNF-6?), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3? and HNF-3? have also been shown to be involved in gastrulation events such as body axis formation.

for HNF-3b / FOXA2 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HNF-3? / FOXA2 antibody.

for HNF-3b / FOXA2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HNF-3b / FOXA2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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