KLF4 antibody (pAb)

by Active Motif
Available SKUs: 39745 |
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for KLF4 antibody (pAb)Application Notes

Validated Applications:
WB: 1:2,000 - 1:5,000 dilution

Published Applications: see references

for KLF4 antibody (pAb)Immunogen

This KLF4 antibody was raised against recombinant full-length human KLF4.

for KLF4 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for KLF4 antibody (pAb)References

KLF4 antibody (pAb) tested by Western blot.

KLF4 antibody tested by Western blot.
Lane 1: Nuclear extract (20 µg) of Hct116 cells.
Lane 2: Nuclear extract (20 µg) of primary human keratinocyte cells. Both probed with KLF4 antibody at a 1:5,000 dilution.

for KLF4 antibody (pAb)Background

KLF4 (Kruppel-like factor 4) is a transcription factor that binds GC-rich DNA sequences having a consensus core DNA binding sequence of CACCC. It is a member of the Kruppel-like factor (KLF) family, highly expressed in the gastrointestinal tract, skin and other epithelial tissues. Krüppel-like factors are a family of zinc-finger transcription factors that play important roles in many fundamental biologic processes including development, proliferation, differentiation, and apoptosis. KLF4 can function both as an activator and repressor of transcription, depending on the gene targeted. Generally, KLF4 serves as a cell cycle regulator, and it may be a tumor suppressor. KLF4 also appears to function as an oncogene in some situations. Additionally, overexpression of KLF4 (along with Oct3/4, Sox2 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency.

for KLF4 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for KLF4 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

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