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NF?B p100 antibody (pAb)

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for NFkB p100 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for NFkB p100 antibody (pAb)Immunogen

This NFkB p100 antibody was raised against a peptide derived from the C-terminal region of human NFkB p100.

for NFkB p100 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NFkB p100 antibody (pAb)References

 
NF?B p100 antibody (pAb) tested by Western blot.

NF?B p100 pAb tested by Western blot.
Detection of NF?B p100 by Western blot analysis. NF?B p100 is detected in nuclear extracts derived from Jurkat cells using NF?B p100 rabbit polyclonal antibody at a 1:2,000 dilution.

for NFkB p100 antibody (pAb)Background

NF?B p100 (Nuclear factor NF-kappa-B p100 subunit also known as NF?B2 p100) is a member of the NFkB family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The family also includes c-Rel, RelA (NF?B p65, NF?B3) and RelB (I-Rel). Rel proteins form dimers with the NF?B p50 (proteolytically processed from p105/NF?B1) and p52 (proteolytically processed from p100/NF?B2) members of the NF?B family that bind DNA and activate transcription. The NF?B p65/p50 heterodimer is the most abundant form of NF?B.

NF?B signaling is negatively regulated by the sequestration of the NF?B complex in the cytoplasm by its association with the I?B family of inhibitory proteins. The I?B Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NF?B. The IKK complex is composed of two catalytic subunits (IKK? and IKK?) and a regulatory subunit (IKK?). Upon its activation, the IKK complex phosphorylates I?B proteins (I?B?, I?B? phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NF?B complex to translocate to the nucleus where it regulates transcription by binding to DNA.

for NFkB p100 antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for NF?B p100 pAb.

for NFkB p100 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NFkB p100 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting