Pht1 / Histone H2A.Z antibody (pAb), sample
for Pht1 / Histone H2A.Z antibody (pAb) (H2AZ)Application Notes
WB: 1:500 - 1:1,000 dilution
for Pht1 / Histone H2A.Z antibody (pAb) (H2AZ)Immunogen
This Pht1 / H2AZ antibody was raised against a peptide corresponding to the C-terminus of Pht1, the histone H2A variant in S. pombe.
for Pht1 / Histone H2A.Z antibody (pAb) (H2AZ)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for Pht1 / Histone H2A.Z antibody (pAb) (H2AZ)References
Pht1 is the S. pombe homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture. It is also implicated in suppressing the accumulation of anti-sense transcripts through interaction with RNAi machinery and heterochromatin proteins.
for Pht1 / Histone H2A.Z antibody (pAb) (H2AZ)Positive Control
Whole-cell extracts from S. pombe can be used as a positive control for Pht1 / H2AZ antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for Pht1 / Histone H2A.Z antibody (pAb) (H2AZ)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting