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Histone H3S10ph antibody (mAb)

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for Histone H3S10ph antibody (mAb) (Clone 6G8B7)Application Notes

Validated Applications:
ICC/IF: 0.5 - 2 µg/ml dilution
WB: 0.2 - 2 µg/ml dilution
ELISA: Individual optimization required.

for Histone H3S10ph antibody (mAb) (Clone 6G8B7)Immunogen

This antibody was raised against a peptide corresponding to amino acids 1-19 including phospho-serine 10 of human Histone H3.

for Histone H3S10ph antibody (mAb) (Clone 6G8B7)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3S10ph antibody (mAb) (Clone 6G8B7)References

Histone H3S10ph antibody (mAb) tested by immunofluorescence.

Histone H3S10ph antibody (mAb) (Clone 6G8B7) tested by immunofluorescence.
Top: HeLa cells stained with H3S10ph antibody (mAb) at a dilution of 0.2 ?g/ml. Bottom: Merge with Hoechst.

Histone H3S10ph antibody (mAb) tested by Western blot.

Histone H3S10ph antibody (mAb) (Clone 6G8B7) tested by Western blot.
HeLa nuclear extract (20 µg) probed with Histone H3S10ph antibody (mAb) at a dilution of 2 µg/ml.

Histone H3S10ph antibody (mAb) tested by Histone Peptide Array

Histone H3S10ph antibody (mAb) (Clone 6G8B7) specificity tested by Peptide Array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3S10ph antibody (mAb) was applied to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001) at a dilution of 0.3 µg/ml. The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Recognition of the H3 serine 10 peptides by the antibody may be blocked by modifications at arginine 8, lysine 9, threonine 11 or lysine 14.

Histone H3S10ph antibody (mAb) tested by ELISA.

Histone H3S10ph antibody (mAb) (Clone 6G8B7) tested by ELISA.
BSA conjugated peptides were probed to show the specificity of the H3S10ph antibody (mAb). The range of antibody dilution used is as shown.

for Histone H3S10ph antibody (mAb) (Clone 6G8B7)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is strongly phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3S10ph antibody (mAb).

for Histone H3S10ph antibody (mAb) (Clone 6G8B7)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3S10ph antibody (mAb) (Clone 6G8B7)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting