STAT1 phospho Ser727 antibody (pAb), sample
for STAT1 phospho Ser727 antibody (pAb)Application Notes
WB: 1:500 - 1:1,000 dilution
for STAT1 phospho Ser727 antibody (pAb)Immunogen
This STAT1 phospho Ser727 antibody was raised against a synthetic peptide containing phospho-serine 727 of human STAT1.
for STAT1 phospho Ser727 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
for STAT1 phospho Ser727 antibody (pAb)References
STAT1 is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. STAT1 is alternatively spliced to give rise to STAT1? and STAT1?, both of which mediate interferon ? activity, but only STAT1? mediates interferon-? activity. STAT1 plays a critical role in various forms of cell death, both apoptotic and non-apoptotic. The mechanisms of action are both by transcriptional activation of target genes, and by direct association with apoptotic regulators, including TRADD, p53, and HDAC (HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6 and HDAC11). STAT1 target genes include the pro-inflammatory genes for iNOS, COX, VCAM, and ICAM, making STAT1 an attractive anti-inflammatory target.
for STAT1 phospho Ser727 antibody (pAb)Positive Control
Whole-cell extracts from HeLa cells treated with IFN? (2500 U/ml for 30 minutes) can be used as a positive control for STAT1 phospho Ser727 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for STAT1 phospho Ser727 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting