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FOXO1/FKHR antibody (pAb)

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for FOXO1/FKHR antibody (pAb)Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution

for FOXO1/FKHR antibody (pAb)Immunogen

This FOXO1/FKHR antibody was raised against a peptide from the C-terminal region of human FOXO1/FKHR.

for FOXO1/FKHR antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for FOXO1/FKHR antibody (pAb)References

FOXO1/FKHR antibody (pAb) tested by immunoprecipitation.

FOXO1/FKHR antibody (pAb) tested by Immunoprecipitation.
10 µl of FOXO1/FKHR antibody was used to immunoprecipitate FOXO1/FKHR from 500 µg of K562 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FOXO1/FKHR antibody at a dilution of 1:500.

FOXO1/FKHR antibody (pAb) tested by Western blot.

FOXO1/FKHR antibody (pAb) tested by Western blot. U2OS nuclear extract (20 µg per lane) was probed with 39629 at a 1:500 dilution.

for FOXO1/FKHR antibody (pAb)Background

FOXO1/FKHR (forkhead box 01) belongs to the forkhead family of transcription factors that contain a distinct forkhead domain. Chromosomal aberrations involving FOXO1, such as translocation with PAX3, are associated with aveolar rhabdomyosarcoma. The specific function of this gene is undetermined.

for FOXO1/FKHR antibody (pAb)Positive Control

Nuclear extracts from U2OS cells can be used as a positive control for FOXO1/FKHR antibody.

for FOXO1/FKHR antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for FOXO1/FKHR antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting